流通池介质交换差示脉冲阳极溶出伏安法用于多元素重叠峰的分离及其同时测定

本文用流通池介质交换差示脉冲阳极溶出伏安法在盐酸介质中富集,而以碳酸钠-碳酸氢钠缓冲溶液为溶出介质,有效地分离了酸性介质中Cu(Ⅱ)、Sb(Ⅲ)、Pb(Ⅱ)、Tl(Ⅰ)、Cd(Ⅱ)、In(Ⅲ)的重叠溶出峰,实现了多离子的同时测定。     

8-羟基喹啉-5-磺酸纤维微柱分离富集电感耦合等离子体原子发射光谱法同时测定多种痕量元素

本文合成了8-羟基喹啉-5-磺酸纤维滤纸片,以此做为微柱填充物同时富集了10种痕量元素,并分离了样品中的高盐组分.本方法可使痕量组分的分离与富集同步进行,因而特别适用于原子光谱测定前样品的预处理.     

Synthesis of monodisperse poly(chloromethylstyrene-co-divinylbenzene) beads and their application in separation of biopolymers

The monodisperse, porous poly(chloromethylstyrene-co-divinylbenzene) beads of 7.9 microm were prepared by a single-step swelling and polymerization method. The seed particles prepared by dispersion polymerization exhibited good absorption of the monomer phase. Based on this media, a weak cation-exchange (WCX) stationary phase for HPLC was synthesized by a new chemically modified method. The prepared resin has advantages of biopolymer separation, high column efficiency, low column backpressure, high protein mass recovery, and good resolution for proteins. The dynamic protein-loading capacity of the synthesized WCX packings was 18.2 mg/g. Five proteins were separated in 3.0 min using the synthesized WCX stationary phase. The experimental results show that the obtained WCX resin has very weak hydrophobicity. The WCX resin was also used for the rapid separation and purification of lysozyme from egg white in 5.0 min with only one step. The purity and specific bioactivity of the purified lysozyme were found to be more than 93% and 70 245 U/mg, respectively.     

钠硼硅酸盐玻璃分相及浸析的NMR研究

用多种ＮＭＲ方法，包括＾２９Ｓｉ、＾２３Ｎａ，＾１１Ｂ ＭａＳ及＾２９Ｓｉ ＣＰ／ＭＡＳ ＮＭＲ，研究了一定组成的ａ２Ｏ－Ｂ２Ｏ３－ＳｉＯ２三元体系玻璃在不同温度下分相处理２４小时及用酸浸析处理后的结构变化。结果表明，在低于分相上界临界温度的范围内，存在着一个温度转折点，低于这一温度时，受动力学因素影响，分相未达平衡态，温度愈低，距平衡态愈过，分相愈下完全。超过这一温度，分相可达平衡态，受热力学控     

氨基羧酸纤维分离富集-电感耦合等离子体原子发射光谱法测定多种痕量稀土元素

本文制备了氨基羧酸纤维滤纸片作为柱填充物，成功地分离和富集了地化样品中的多种稀土元素。富集后的稀土元素采用电感耦合等离子体原子发射光谱法测定，回收率为90％～109％。本文还对基体干扰及其消除进行了研究。     

Gas-stirred solvent extraction with propylene carbonate for separation and direct polarographic determination of thallium (III)

Gas-stirred propylene carbonate (PC) extraction can be performed by bubbling nitrogen without loss of volume. This extraction technique has proved to be highly useful for the separation and subsequent direct polarographic estimation of Tl(III) in the recovered PC phase. Tl(III) can be determined by differential-pulse polarography over the concentration range of 0.4 10 g ml^–1 without any interferences due to most metal ions such as Pb(II), Bi(III), Cu(II), Sb(III) and As(III). This proposed technique would be possible to use as a simple extraction step prior to the final estimation by instrumental method of analysis such as atomic absorption spectrometry or spectrophtometry.     

Separation of Anions of Organic Acids in Concentrated Solutions by Means of Capillary Isotachophoresis

The separation of a mixture containing anions of nine organic acids is investigated on an instrument for capillary isotachophoresis with an optical detector for the registration of boundaries. The separation is conducted in concentrated solutions of sodium salts. A counterflow is used during the separation.     

毛细管电泳法快速分离和检测肠毒性大肠杆菌

 建立了快速分离和检测引起仔猪腹泻的肠毒性大肠杆菌K88、K99和 987P细菌细胞的毛细管区带电泳方法 ,并进行了腹泻仔猪粪便中肠毒性大肠杆菌的应用检测分析。结果表明 ,在电泳缓冲液为 0 0 5mol/LNa2 CO3 NaHCO3(pH 9 9)、分离电压为 1 4 1kV、检测波长为 2 1 0nm的电泳条件下 ,E coliK88、K99和 987P的细胞分别具有单一、稳定的特征谱峰 ,其保留时间的相对标准偏差RSD≤ 0 9% ;在确定的实验条件下 ,实现了腹泻仔猪粪便中肠毒性大肠杆菌的快速检测分析 ,发现将出生 5d～ 6d的腹泻仔猪粪便引入培养基中增殖后主要检出K88。     

毛细管电泳法测定左旋多巴药片的光学纯度

建立了一种毛细管电泳测定左旋多巴药片光学纯度的新方法。D,L-多巴(D,L-dopa)用2,4-二硝基氯苯(CDNB)衍生,以β-环糊精(-βCD)为手性选择剂,在硼砂(pH 9.0)缓冲溶液中进行毛细管电泳手性分离。采用紫外检测,测定波长为220 nm。在测定条件下,对映体分离在9 m in内完成。L-dopa中D-dopa的含量在0.10%~2.0%范围内,与D-dopa的峰面积呈现良好的线性关系。在L-dopa中加入不同量的D-dopa,测得回收率在96%~102%之间,每个样品分别测定3次,其相对标准偏差(RSD)小于2.3%。方法用于市售左旋多巴药片的分析,测得左旋多巴药片中D-dopa的含量为0.20%。     

酯化交联聚乙烯醇膜用于渗透气化法分离EtoH／H2O的研究

合成了一系列磷酸和二元羧酸酯化交联改性的聚乙烯醇(PVA)膜,研究了它们用于水-乙醇混合液的渗透气化分离规律。磷酸改性的PVA膜具有较大的通量,当料液浓度为50%时,通量可达1200g/m~2·hr.,分离系数α_(H_2O/EtOH)=10。丙二酸改性PVA膜在料液浓度为50%时,通量可达800g/m~2·hr.,且α_(H_2O/EtOH)=18。并作了这两种膜的通量和分离系数随料液浓度的变化曲线。草酸交联的PVA膜具有较高的分离系数,但通量很小。求出了水、乙醇及总的表现渗透活化能。